Testicular Sperm Aspiration (TESA)
For some patients, ejaculation is impossible, or little or no living or motile sperm can be recovered from their ejaculate. Examples of this include patients with prior vasectomy, patients born with congenital absence of the vas deferens, and with zero sperm count due to other causes. However, these men frequently have sperm in the testicles. Sperm can be harvested directly from the testes through Testicular Sperm Aspiration (TESA) or Testicular Sperm Extraction (TESE). Because a low number of sperm can be collected this way, and because testicular sperm cannot fertilize an egg on its own, TESA must be used along with In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI).How does TESA work?
- Under local sedation, a small needle is used to extract sperm directly from the testicle.
- Testicular sperm must be used with In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI). A single sperm, processed in our laboratory, is injected directly into the oocyte (egg), fertilizing it.
- Once fertilized, the developing embryo is implanted into the uterus.
Cryopreservation - Some patients have a very small amount of motile or living sperm in their semen. To increase the amount available for use in IUI, IVF and ICSI, sperm can be frozen (cryopreserved) and stored at the Reproductive Fertility Center cryobank for use in future fertilizations. Cryopreservation can also be used to store unused semen and sperm samples to prevent the need for further sperm aspiration.

